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[์—ฐ๊ตฌ์†Œ์‹] ๊ฒํŠธ๋Œ€ ๋ฐ•์ง€ํ˜œ ๋ฐ•์‚ฌ, ํ•œ๊ตญ์—ฐ๊ตฌ์žฌ๋‹จ ๊ณผ์ œ ์ˆ˜์ฃผ
18/05/30 10:40 | ๊ดถฤ๋ฆฌ์ž | View 21446 | Comments 0

๊ฒํŠธ๋Œ€ ๋ฐ•์ง€ํ˜œ ๋ฐ•์‚ฌ, ํ•œ๊ตญ์—ฐ๊ตฌ์žฌ๋‹จ ๊ณผ์ œ ์ˆ˜์ฃผ

 

5์›”25์ผ, ๊ฒํŠธ๋Œ€ํ•™๊ต ๊ธ€๋กœ๋ฒŒ์บ ํผ์Šค ์‹๋ฌผ๋ฐ”์ด์˜คํ™œ์„ฑ๋ฌผ์งˆ์—ฐ๊ตฌ์„ผํ„ฐ (ฬวะฤlogoศ๋ฟฺ Plant Bioactive Compound Research Centre) ์˜ Postdoc ๊ณผ์ •์„ ๋ฐŸ๊ณ  ์žˆ๋Š” ๋ฐ•์ง€ํ˜œ๋ฐ•์‚ฌ๊ฐ€ ํ•œ๊ตญ์—ฐ๊ตฌ์žฌ๋‹จ์— ์‹ ์ฒญํ•œ ์—ฐ๊ตฌ๊ณผ์ œ๊ฐ€ ‘2018๋…„ ์ด๊ณตํ•™๊ฐœ์ธ๊ธฐ์ดˆ์—ฐ๊ตฌ์ง€์›์‚ฌ์—…’์— ์„ ์ •๋˜์—ˆ๋‹ค. ์ด๋กœ์„œ ฬวะฤlogoศ๋ฟฺ ์‚ฌ์ƒ ์ตœ์ดˆ๋กœ ๊ฐœ์ธ์—ฐ๊ตฌ์ž๊ฐ€ NRF๊ณผ์ œ์ˆ˜์ฃผ์— ์„ฑ๊ณตํ•œ ์‚ฌ๋ก€๊ฐ€ ํƒ„์ƒ๋˜์˜€๋‹ค. ํ•œ๊ตญ์—ฐ๊ตฌ์žฌ๋‹จ ์ด๊ณตํ•™๊ฐœ์ธ๊ธฐ์ดˆ์—ฐ๊ตฌ์ง€์›์‚ฌ์—…์˜ ๋ชฉ์ ์€ ์ด๊ณตํ•™๋ถ„์•ผ ํ’€๋ฟŒ๋ฆฌ ๊ฐœ์ธ๊ธฐ์ดˆ์—ฐ๊ตฌ๋ฅผ ํญ๋„“๊ฒŒ ์ง€์›ํ•˜์—ฌ ๋ณ€ํ˜์  ์—ฐ๊ตฌ๊ธฐ๋ฐ˜์„ ํ™•๋Œ€ํ•˜๊ณ  ๊ตญ๊ฐ€ ์—ฐ๊ตฌ์—ญ๋Ÿ‰์„ ์ œ๊ณ ํ•˜๋ฉฐ, ์—ฐ๊ตฌ์ž๊ฐ€ ์•ˆ์ •์ ์œผ๋กœ ์—ฐ๊ตฌ์— ๋ชฐ์ž…ํ•  ์ˆ˜ ์žˆ๋Š” ํ™˜๊ฒฝ์„ ์กฐ์„ฑํ•˜์—ฌ ์ฐฝ์˜์  ์—ฐ๊ตฌ๋ฅผ ํ™œ์„ฑํ™”์‹œํ‚ค๋Š” ๊ฒƒ์ด๋‹ค. 2018๋…„๋„ ์ตœ์ข… ์„ ์ • ํ˜„ํ™ฉ์œผ๋กœ๋Š” ๊ธฐ๋ณธ ์—ฐ๊ตฌ 2,408๊ฑด, ๋ณดํ˜ธ ์—ฐ๊ตฌ 7๊ฑด, ์ง€์—ญ๋Œ€ํ•™์šฐ์ˆ˜๊ณผํ•™์ž 238๊ฑด, ํ•œ๊ตญํ˜•SGER 98๊ฑด, ๊ธฐ๋ณธ ์—ฐ๊ตฌ(ํ›„์†) 7๊ฑด, ๋ฐ ์ง€์—ญ๋Œ€ํ•™์šฐ์ˆ˜๊ณผํ•™์ž(ํ›„์†) 19๊ฑด์ด๋‹ค.

 

์ด๋ฒˆ ํ•œ๊ตญ์—ฐ๊ตฌ์žฌ๋‹จ์— ์ฑ„ํƒ๋œ ๊ณผ์ œ ์ œ๋ชฉ์€ ‘๊ฐฏ๋ฒŒ ์ €์„œ์„ฑ ๊ธฐํ˜• ๋ฐ ์ง€์งˆ์ฒด ํ˜•์„ฑ ๊ทœ์กฐ๋ฅ˜์˜ ๋ถ„์žํ™”ํ•™์ƒ๋ฌผ๋‹ค์–‘์„ฑ๊ณผ ์ƒ๋ฆฌ์ƒํƒœํ•™์  ๊ธฐ์ดˆ ์—ฐ๊ตฌ (A basic study on molecular chemobiodiversity and ecophysiology of periphytic diatoms (showing deformity and lipid body formation) collected from a tidal-flat ecosystem) ’ ์ด๋ฉฐ, ๋ฐ•์ง€ํ˜œ ๋ฐ•์‚ฌ๋Š” ์ด ์—ฐ๊ตฌ๊ณผ์ œ๋ฅผ ํ†ตํ•ด, ์™ธ๊ตญ๋Œ€ํ•™์ด ๊ตญ๋‚ด์—์„œ ๊ตญ๊ฐ€๊ณผ์ œ๋ฅผ ์‹ ์ฒญํ•˜๋Š” ๋ฐ ๊ฒช๊ณ  ์žˆ๋Š” ์–ด๋ ค์šด ์ƒํ™ฉ์—์„œ ๋ณธ์ธ์˜ ๊ณผ์ œ๊ฐ€ ฬวะฤlogoศ๋ฟฺ ์—ฐ๊ตฌ์— ๊ธฐ์—ฌ๊ฐ€ ๋˜๊ธฐ๋ฅผ ๋ฐ”๋ž€๋‹ค๋Š” ์†Œ๊ฐ์„ ๋ฐํ˜”๋‹ค.

 

๋ฐ•์ง€ํ˜œ ๋ฐ•์‚ฌ๋Š” 2017๋…„ 2์›”์— ๊ฒํŠธ๋Œ€ํ•™๊ต ๊ธ€๋กœ๋ฒŒ์บ ํผ์Šค์™€ ์ธ์ฒœ๋Œ€์—์„œ ์ฒซ ๋ฒˆ์งธ๋กœ ๊ณต๋™ ๋ฐ•์‚ฌํ•™์œ„๋ฅผ ๋ฐ›์•˜์œผ๋ฉฐ, ์ฃผ ์—ฐ๊ตฌ์˜์—ญ์€ ๋ถ„์ž์‹๋ฌผํ•™์œผ๋กœ ํ•ด์กฐ๋ฅ˜ ์„ธํฌ ๋ฐ ๋ถ„์ž๋งค์ปค๋‹ˆ์ฆ˜์— ๋Œ€ํ•œ ์—ฐ๊ตฌ๋ฅผ ํ†ตํ•ด ํ•ด์–‘ํ™˜๊ฒฝ๋ณดํ˜ธ์™€ ๋ฐ”์ด์˜ค์†Œ์žฌ์˜ ์ง€์† ๊ฐ€๋Šฅํ•œ ์‘์šฉ์ด๋‹ค. ๋ฐ•์ง€ํ˜œ ๋ฐ•์‚ฌ์˜ ํŒŒ๋ž˜์— ๋Œ€ํ•œ ์—ฐ๊ตฌ๊ฒฐ๊ณผ๋Š” ์ตœ๊ทผ์— Elsevier ์ถœํŒ์˜ ๋ถ ์ฑ•ํ„ฐ์— ์ €์ˆ ๋˜์—ˆ๋‹ค.

 

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๋ฐ•์ง€ํ˜œ ๋ฐ•์‚ฌ์˜ ์ตœ๊ทผ ๋ฐœํ‘œ๋œ ๋ถ ์ฑ•ํ„ฐ ๊ฐœ์š”:

๊ตฌ๋ฉ๊ฐˆํŒŒ๋ž˜ (U. purtusa)๋Š” ์ง€์ค‘ํ•ด, ํƒœํ‰์–‘ ๋ฐ ์ธ๋„์–‘์— ๋„๋ฆฌ ๋ถ„ํฌํ•˜๋Š” ๊ฐˆํŒŒ๋ž˜๋ฅ˜ (Ulva spp.)์— ์†ํ•˜๋Š” ๋…น์กฐ๋ฅ˜์ด๋‹ค. ์ตœ๊ทผ, ์‚ฐ์—…ํ™œ๋™ ๋ฐ ๋„์‹ฌ์œผ๋กœ๋ถ€ํ„ฐ ์œ ๋ž˜ํ•˜๋Š” ์œ ๋…์„ฑ ํ๊ธฐ๋ฌผ์ด ๋นˆ๋ฒˆํ•˜๊ฒŒ ์—ฐ์•ˆ ํ•ด์—ญ์— ์นจ๋ฒ”ํ•˜๋Š”๋ฐ, ๊ฐˆํŒŒ๋ž˜๋Š” ์ด๋ฅผ ํƒ์ง€ํ•  ์ˆ˜ ์žˆ๋Š” ์ค‘์š”ํ•œ ์ง€ํ‘œ์ข…์œผ๋กœ ๋ณด๊ณ ๋œ ๋ฐ” ์žˆ๋‹ค. ๊ฐˆํŒŒ๋ž˜๋Š” ์—ฐ์•ˆ ์ƒํƒœ๊ณ„์˜ ๊ธฐ์ดˆ๋ฅผ ๊ตฌ์„ฑํ•˜๋Š” ์ผ์ฐจ ์ƒ์‚ฐ์ž๋กœ์„œ ๋‹ค๋ฅธ ํ•ด์–‘ ์ƒ๋ฌผ์—๊ฒŒ ์„œ์‹์ง€๋ฅผ ์ œ๊ณตํ•˜๊ณ , ์‹ํ’ˆ ๋ฐ ์˜์•ฝํ’ˆ์˜ ์ค‘์š”ํ•œ ์ฒœ์—ฐ ์ž์›์œผ๋กœ ํ™œ์šฉ๋  ์ˆ˜ ์žˆ๋‹ค. ์ƒ์‹์€ ๊ฐœ์ฒด๊ตฐ์˜ ์œ ์ง€์— ํ•„์ˆ˜์ ์ธ ๊ณผ์ •์œผ๋กœ์„œ ์ƒ์‹ ๊ณผ์ •์— ๋ฌธ์ œ๊ฐ€ ์ƒ๊ธฐ๋ฉด ์ด๋Š” ๊ณง ๊ฐœ์ฒด๊ตฐ์˜ ์‚ฌ๋ฉธ์„ ์˜๋ฏธํ•˜๊ณ  ์—ฐ์‡„์ ์œผ๋กœ ์ƒ๋ฌผ๊ตฐ์ง‘ ์ „์ฒด์— ๋ณ€ํ™”๋ฅผ ์ดˆ๋ž˜ํ•˜๊ฒŒ ๋œ๋‹ค. ๋งŽ์€ ๊ฐˆํŒŒ๋ž˜๋Š” ์ง€๋ฆฌ์ ์œผ๋กœ ๋„“์€ ๋ฒ”์œ„๋กœ ๋ถ„ํฌํ•˜๊ณ  ์žˆ์œผ๋ฉฐ ์œ ์‚ฌํ•œ ํ˜•ํƒœ์˜ ์ƒ์‹ ๊ณผ์ •์„ ๊ฐ€์ง„๋‹ค. ๋”ฐ๋ผ์„œ, ๋ณธ ํ‘œ์ค€ ์‹œํ—˜ ๋ฐฉ๋ฒ•์€ ์ „ ์„ธ๊ณ„ ๋Œ€๋ถ€๋ถ„ ์—ฐ์•ˆ ํ•ด์—ญ์—์„œ ๋ณด๊ณ ๋œ ๋™์ผํ•œ ์†(genus)์— ์†ํ•˜๋Š” ๋‹ค๋ฅธ ์ข…(species)์— ์ ์šฉ๋  ๊ฐ€๋Šฅ์„ฑ์ด ์žˆ๋‹ค. ์—ฌ๊ธฐ์„œ ์ œ์•ˆํ•˜๋Š” ํŒŒ๋ž˜๋ฅผ ์ด์šฉํ•œ ์ƒํƒœ๋…์„ฑ ๊ธฐ๋ฒ•์€ ์ข…๋ž˜์— ์‚ฌ์šฉ๋˜๋Š” ๊ธฐ์ˆ ์— ๋น„ํ•ด ๋งŽ์€ ์‹ค์งˆ์ ์ธ ์ด์ ์„ ์ œ๊ณตํ•œ๋‹ค. ๋ช‡ ๊ฐ€์ง€ ์˜ˆ๋ฅผ ๋“ค์ž๋ฉด, ์ „๋ฌธ์  ์ง€์‹์ด ์š”๊ตฌ๋˜์ง€ ์•Š๋Š”๋‹ค, 96์‹œ๊ฐ„ ๋…ธ์ถœ์‹œ๊ฐ„์— ์‹œํ—˜ ๋ถ„์„์€ 3์‹œ๊ฐ„ ์ •๋„ ์†Œ์š”๋˜๋ฉฐ, ํ•„์š”ํ•œ ์‹œ๋ฃŒ์˜ ์–‘์€ ๋งค์šฐ ์ ๊ณ  ์‹œํ—˜ ์šฉ๊ธฐ๋„ ์›ฐ ํ”Œ๋ ˆ์ดํŠธ๋งŒ ์š”๊ตฌ๋˜๊ธฐ ๋•Œ๋ฌธ์— ๋น„์šฉ์ ์œผ๋กœ๋„ ํšจ์œจ์ ์ธ ๋ฐฉ๋ฒ•์ด๋ผ๊ณ  ํ•  ์ˆ˜ ์žˆ๋‹ค. ๊ตฌ๋ฉ๊ฐˆํŒŒ๋ž˜ ์‹œํ—˜๋ฒ•์˜ ๋ฏผ๊ฐ๋„๋Š” ์ผ๋ฐ˜์ ์œผ๋กœ ์ด์šฉ ๊ฐ€๋Šฅํ•œ ๋˜๋Š” ์ž˜ ํ™•๋ฆฝ๋œ ์ƒ๋ฌผ๊ฒ€์ •๋ฐฉ๋ฒ•๊ณผ ์œ ์‚ฌํ•˜๋ฉฐ, ๋งŽ์€ ๊ฒฝ์šฐ์— ์žˆ์–ด์„œ ๊ธฐ์กด์˜ ๋ฐฉ๋ฒ•๋ณด๋‹ค ๋”์šฑ ์šฐ์ˆ˜ํ•˜๋ฉฐ, ์ƒ์‹์€ ์ƒ๋ฌผ์˜ ๋ฒˆ์‹์ด ์ด‰์ง„๋˜๋Š” ์ˆ˜๋‹จ์ด๋ฏ€๋กœ ์ƒํƒœํ•™์ ์œผ๋กœ ๋งค์šฐ ์ค‘์š”ํ•œ ์ข…๋ง์ ์ด๋ผ๊ณ  ํ•  ์ˆ˜ ์žˆ๋‹ค. ๋˜ํ•œ, ์•ผ์™ธ์—์„œ ์ฑ„์ง‘๋œ ๊ฐˆํŒŒ๋ž˜๋Š” ์‰ฝ๊ฒŒ ์‹ค๋‚ด ๋ฐฐ์–‘์„ ์œ ์ง€ํ•  ์ˆ˜ ์žˆ๊ณ  ์‹คํ—˜์‹ค์—์„œ ์ธ๊ณต ์ƒ์‹ ์œ ๋„๋ฅผ ํ•  ์ˆ˜ ์žˆ๋‹ค. ๊ตฌ๋ฉ๊ฐˆํŒŒ๋ž˜ ์‹œํ—˜๋ฐฉ๋ฒ•์€ ์—ฐ์•ˆ ๋ฐ ๊ธฐ์ˆ˜์—ญ ์ƒํƒœ๊ณ„๋ฅผ ๋ชจ๋‹ˆํ„ฐ๋งํ•˜๊ธฐ ์œ„ํ•ด ๊ธฐ์กด์— ์‚ฌ์šฉ๋˜๋Š” ๋ฐฉ๋ฒ•์— ์ถ”๊ฐ€์ ์œผ๋กœ ์ ์šฉํ•  ์ˆ˜ ์žˆ๋Š” ๊ฐ€์น˜ ์žˆ๋Š” ๋ฐฉ๋ฒ•์ด ๋  ์ˆ˜ ์žˆ๋‹ค. ๋ณธ ์‹œํ—˜๋ฐฉ๋ฒ•์€ ํ์ˆ˜์— ๋Œ€ํ•œ TIE/TRE ์ ˆ์ฐจ์—๋„ ์ ์šฉ๋  ์ˆ˜ ์žˆ๋‹ค. ์ด ๊ธฐ์ˆ ์€ ๊ธฐ์กด์— ์ œ์•ˆํ•œ ISO ๋ฐฉ๋ฒ•์„ ๊ธฐ๋ฐ˜์œผ๋กœ ๊ฐœ๋ฐœ๋˜์—ˆ๊ธฐ ๋•Œ๋ฌธ์— ํ์ˆ˜ ๊ด€๋ฆฌ ๊ธฐ์ˆ ์—๋„ ์‰ฝ๊ฒŒ ์‘์šฉ๋˜์–ด ๊ฐœ๋ฐœ๋  ์ˆ˜ ์žˆ์œผ๋ฉฐ, ๋ณด๋‹ค ๊ฐ„ํŽธํ•œ ์‚ฌ์šฉ์„ ์œ„ํ•ด Ulvaํ‚คํŠธ์™€ ์ž๋™ํ™”๊ธฐ๊ธฐ์ธ UlvaTOX๋„ ๊ฐœ๋ฐœ๋œ ๋ฐ” ์žˆ๋‹ค.

 

Ulva pertusa Kjellman is reported from the Mediterranean, Pacific, and Indian Oceans. Ulva spp. are important representatives of macroalgal communities in coastal waters that are under threat from the frequent inundations of toxic waste derived from industrial and municipal sources. These algae are primary producers constituting the basis of costal ecosystem and, moreover, provide shelter and habitats for other marine organisms; they are also important natural sources of food and pharmaceutical products. Reproduction is a critical process by which populations perpetuate, and disturbance of this process can cause failure in recruitment, leading to the disappearance of the population and ultimately to modifications in community structure and dynamics. Members of Ulva have a wide range of geographic distribution and undergo similar reproductive processes. Therefore, the test method presented in this standard has the potential for application to other species belonging to the same genus reported from coastal waters in most parts of the world.

The proposed macroalgal toxicity test provides many practical advantages over other currently employed techniques. No specialist expertise is required. The test is cost- and time effective, since it only requires a cell plate, a small volume of water and takes a total of approximately 3 h to conduct around a 96 h incubation period. The sensitivity of the Ulva method is similar to, and in many cases better than, commonly available or well established bioassay methods, and since reproduction is the means by which population recruitment is facilitated, the measured endpoint being ecologically significant cannot be disputed. In addition, field-collected samples of unialgal Ulva plants can easily be held and acclimated in a holding tank for 1 to 2 month(s), and artificial induction of reproduction in a laboratory is easily achieved using vegetative thalli, allowing all year round testing to be performed. The Ulva reproduction bioassay would be a valuable addition to the existing suite of tests routinely used to monitor coastal and estuarine ecosystems.

The Ulva test can also be applicable to the TIE/TRE procedures for waste waters. The TIE/TRE technique using Ulva may signify a break-through in waste-water management technology since no such technique has been developed so far based on the established ISO methods.

The Ulva-Kit and automated UlvaTOX have been developed to facilitate the use of the Ulva method.

 

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